Psh4x 8bp [repack] -
A 2024 preprint demonstrated that gRNAs containing the psh4x 8bp motif at the 5' end reduced off-target cleavage by 78% without compromising on-target activity.
A 2024 preprint demonstrated that gRNAs containing the psh4x 8bp motif at the 5' end reduced off-target cleavage by 78% without compromising on-target activity.